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anti fis1 antibody (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc anti fis1 antibody
Anti Fis1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+fis1/FIS1+Rabbit+mAb/pmc13006403-10-0-3
Average 95 stars, based on 61 article reviews

anti fis1 antibody - by Bioz Stars, 2026-09

95/100 stars

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Electrophoresis:

Article Title: Facilitating Pro-survival Mitophagy for Alleviating Parkinson's Disease via Sequence-Targeted Lycopene Nanodots.
Article Snippet: The BCA kit (Solarbio Biotechnology Co., Ltd) was utilized to equalize the protein concentration of each sample. .. After the different proteins were separated by electrophoresis and transferred to PVDF membranes, which were subsequently blocked in milk for the incubation with primary antibodies overnight including rabbit anti-PINK1 (Abcam, ab216144), rabbit antiParkin (Cell Signaling Technology, CST, 32833S), rabbit anti-LC3B (Abcam, ab192890), rabbit anti-Rab10 (Abcam, ab237703), rabbit anti-Tyrosine hydroxylase (Abcam, ab137869), rabbit anti-α-Synuclein (Abclonal, A20407), rabbit anti-SLC18A2 (Abclonal, A2799), rabbit anti-LAMP1 (Abcam, ab278043), rabbit anti-p62 (Abcam, ab109012), rabbit anti-Beclin1 (Abcam, ab207612), rabbit anti-VDAC1 (Abclonal, A19707), rabbit anti-MFN2 (Abcam, ab124773), rabbit anti-Fis1 (CST, 32525S), rabbit anti-DRP1 (Abcam, ab184247), rabbit anti-phospho-Akt (Ser473)(CST, 4060T), rabbit anti-Bad (CST, 9239T), rabbit anti-Bax (CST, 2772T), rabbit anti-Bcl-2 (CST, 3498T), rabbit anti-Bcl-xL (CST, 2764T), rabbit anti-cleaved Caspase-3 (Asp175)(CST, 9664T), rabbit anti-Heme Oxygenase 1 (Abcam, ab68477), rabbit anti-NRF2 (CST, 12721), rabbit anti-Keap1 (CST, 8047) and rabbit anti-β-Actin (CST, 4970) at 1000 dilutions. .. After washing for 3 times, cleaned membranes were incubated with the secondary antibody (CST, 7074S) at room temperature for further chemiluminescent band imaging.

Incubation:

Western Blot:

Article Title: CD137 Signaling Mediates Pulmonary Artery Endothelial Cell Proliferation Under Hypoxia By Regulating Mitochondrial Dynamics.
Article Snippet: Altered mitochondrial dynamics affect pulmonary artery endothelial cells (PAECs) proliferation, contributing to the development of pulmonary hypertension.. CD137 signaling promotes mitochondrial fission.. We hypothesize CD137 signaling is involved in the excessive proliferation of PAECs.

Immunohistochemistry:

other:

Article Title: Roxadustat (FG-4592) Facilitates Recovery From Renal Damage by Ameliorating Mitochondrial Dysfunction Induced by Folic Acid.
Article Snippet: Rabbit anti-Mfn1 (14739s), rabbit anti-Opa1 (67589s), rabbit anti-Fis1 (86668s), rabbit anti-Drp1 (8570s), rabbit anti-IL-1β (12703s), rabbit anti-CD3 (26582s), rabbit anti-Myeloperoxidase (MPO+) (14569T), rabbit anti-F4/80 (30325s), and rabbit anticollagen I (72026T) antibodies were taken from CST; mouse antiβ-actin (ab8226), rabbit anti-HIF-1α (ab216842), mouse antiPCNA (ab29), rabbit anti-E-cadherin (ab76319), rabbit anti-NCC (ab203674), rabbit anti-α-SMA (ab5694), mouse anti-vimentin (ab92547), rabbit anti-GPX-4 (ab125066), and rabbit antifibronectin (Fn) (ab2413) antibodies were obtained from Abcam; rabbit anti-TNF-α (7B8A11) antibody was purchased from Proteintech; rabbit anti-ZO-1 (PA524716), rabbit antiAQP1 (101AP), and rabbit anti-AQP2 (201AP) antibodies were obtained from Invitrogen; anti-IL-33 (AF3626) antibody was acquired from R&D Systems.

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$Group comparison of mitochondrial Drp1 ( A ) and Fis1 ( B ) expressions. Statistical significance was determined by ANOVA followed by Bonferroni’s post hoc test. Data are shown as mean ± SEM; ns, p = 0.2021 for Drp1; *, p < 0.05 ( p = 0.0041) for Fis1 ( n = 5–7 per group). Representative immunoblots demonstrating Drp1 and Fis1 protein levels in mitochondrial fractions and total cell lysates of AC16 cells infected with Ad.GFP or Ad.GRK2-C340S under normoxic or hypoxia/reoxygenation (H/R) conditions. VDAC served as a mitochondrial loading control and GAPDH served as a total protein marker. Densitometric analysis was performed using Li-Cor Image Studio software and normalized to the respective loading controls. All samples included in each comparative analysis were run on the same gel. Cropped blots are presented for clarity; uncropped blots with molecular weight markers are provided in .$

Journal: Cells

Article Title: Cys340Ser Mutation Abolishing S-Nitrosylation Drives GRK2 Mitochondrial Localization and Dysfunction

doi: 10.3390/cells15050458

Figure Lengend Snippet: Group comparison of mitochondrial Drp1 ( A ) and Fis1 ( B ) expressions. Statistical significance was determined by ANOVA followed by Bonferroni’s post hoc test. Data are shown as mean ± SEM; ns, p = 0.2021 for Drp1; *, p < 0.05 ( p = 0.0041) for Fis1 ( n = 5–7 per group). Representative immunoblots demonstrating Drp1 and Fis1 protein levels in mitochondrial fractions and total cell lysates of AC16 cells infected with Ad.GFP or Ad.GRK2-C340S under normoxic or hypoxia/reoxygenation (H/R) conditions. VDAC served as a mitochondrial loading control and GAPDH served as a total protein marker. Densitometric analysis was performed using Li-Cor Image Studio software and normalized to the respective loading controls. All samples included in each comparative analysis were run on the same gel. Cropped blots are presented for clarity; uncropped blots with molecular weight markers are provided in .

Article Snippet: The primary antibodies used were as follows: GRK2 (13990-1-AP; ProteinTech, Rosemont, IL, USA), GRK2 (05-465; Sigma Aldrich, St. Louis, MO, USA), Drp1 (8570; Cell Signaling Technology, Danvers, MA, USA), Fis1 (32525; Cell Signaling Technology, Danvers, MA, USA), Mfn1 (14739; Cell Signaling Technology, Danvers, MA, USA), Mfn2 (11925; Cell Signaling Technology, Danvers, MA, USA), Opa1 (80471; Cell Signaling Technology), Pink (6946; Cell Signaling Technology, Danvers, MA, USA), Parkin (4211; Cell Signaling Technology, Danvers, MA, USA), LC3 (3868; Cell Signaling Technology, Danvers, MA, USA), Tom20 (42406; Cell Signaling Technology, Danvers, MA, USA), VDAC1 (sc-390996; Santa Cruz Biotechnology, Dallas, TX, USA), and GAPDH (sc-32233; Santa Cruz Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Comparison, Western Blot, Infection, Control, Marker, Software, Molecular Weight